Heat-Treatment of Bovine Colostrum

Batches (30-L) of first-milking bovine colostrum, inoculated with Mycoplasma bovis (108 cfu/mL), Listeria monocytogenes (106 cfu/mL), Escherichia coli O157:H7 (106 cfu/mL), Salmonella enteritidis (106 cfu/mL), and Mycobacterium avium subsp. paratuberculosis (Map; 103 cfu/mL), were heat-treated at 60°C for 120 min in a commercial on-farm batch pasteurizer system. Duplicate 50-mL subsamples of colostrum were collected at 15-min intervals throughout the heat-treatment process for the purpose of bacterial culture and for measurement of IgG concentration (mg/mL) and antibody activity.

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